ABSTRACT
<p><b>OBJECTIVE</b>To explore the relationship between sexual hormones in semen and germ cell apoptosis in male population.</p><p><b>METHODS</b>Sixty-six infertile patients and thirty fertile males were selected randomly. The levels of folicle stimulating hormone ( FSH), prolactin (PRL), luteinizing hormone (LH), and testosterone (T) in semen were measured by ELISA. Terminal deoxynucleotidyl transferase mediated UTP nick end labeling (TUNEL) was used for the detection of germ cell apoptosis.</p><p><b>RESULTS</b>The levels of FSH, LH, PRL, T in thirty fertile men were (1.63 +/- 0.15) U/L, (2.18 +/- 0.21) U/L, (6.34 +/- 0.30) nmol/L, (1.85 +/- 0.11) nmol/L, respectively, and germ cell apoptosis rate was (4.61 +/- 1.23)%. FSH, LH, PRL, T levels in infertile group were (1.25 +/- 0.18) U/L, (1.76 +/- 0.32) U/L, (5.86 +/- 0.13) nmol/l, (1.45 +/- 0.13) nmol/, respectively, and germ cell apoptosis rate was (18.36 +/- 2.04)%. There were significant differences in all parameters between infertile group and fertile group. The levels of FSH, LH, PRL, T were negatively correlated with germ cell apoptosis rates( r = -0.88, -0.93, -0.90, -0.98). The volume of apoptotic germ cell decreased, and chromatin was compacted to form cell-membrane blebs and apoptotic bodies.</p><p><b>CONCLUSION</b>Low concentration of sexual hormones may increase the apoptosis of germ cells, which can induce male infertility.</p>
Subject(s)
Adult , Humans , Male , Apoptosis , Case-Control Studies , Follicle Stimulating Hormone , Metabolism , Germ Cells , Pathology , Gonadal Steroid Hormones , Metabolism , Infertility, Male , Metabolism , Pathology , Luteinizing Hormone , Metabolism , Prolactin , Metabolism , Semen , Metabolism , Testosterone , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of nitric oxide (NO) on human sperm capacitation and acrosome reaction (AR).</p><p><b>METHODS</b>Different concentrations of sodium nitroprusside (SNP) were added to the sperm suspension from 48 healthy fertile men, and the suspension was incubated in 1 x Earle at 37 degrees C for 1 hour. Progesterone was used to induce AR for 15, 30, 45 and 60 min, and then acid phosphatase (ACP) activity in the suspension before and after capacitation and at different time of AR was measured by p-nitrophenyl sodium phosphate assay. In the meantime, sperm motile parameters were assayed by CASA to observe sperm capacitation and AR.</p><p><b>RESULTS</b>ACP activity and sperm motile parameters increased in the 50 approximately 100 nmol/L NO concentration group, showed no significant variation in the 150 approximately 200 nmol/L group, and decreased in the 250 approximately 300 nmol/L group.</p><p><b>CONCLUSION</b>NO can facilitate sperm capacitation, AR and sperm motile parameters in low concentration and suppress them in high concentration. ACP activity assay of sperm is an objective and reliable method to evaluate sperm capacitation and AR in whole sperm population.</p>
Subject(s)
Adult , Humans , Male , Acid Phosphatase , Metabolism , Acrosome Reaction , Physiology , Dose-Response Relationship, Drug , Nitric Oxide , Physiology , Nitric Oxide Donors , Pharmacology , Nitroprusside , Pharmacology , Sperm Capacitation , Physiology , Sperm Motility , Physiology , SpermatozoaABSTRACT
Objective To observe the morphologic changes of peripheral blood lupus cell in patients with systemic lupus erythematosus and investigate its relationship with disease activity in systemic lupus ery- thematosus.Methods Modified classical blood clotting method to observe the morphological changes of pe- ripheral blood lupus cells in 80 cases with systemic lupus erythematosus.Fifty cases were in active stage,and 30 cases were in stable stage.Comparison of serum autoantibody,complement and SLEDAI were also carried out.Results There was significant association between lupus cells in special morphous and autoantibody, such as anti-double-stranded DNA antibody,anti-nucleosome antibodies,complement C3、C4 and SLEDAI(r= 0.588,P=0.056:r=0.759,P=0.135;r=-0.648,P=0.058;r=-0.589,P=0.057,r=0.686,P